Spermatozoon is an intricate motile cell with a well defined head, neck and tail. The shape of the normal sperm is a reflection of proper spermatogenesis in the testicles. Recent studies have highlighted the importance of sperm morphology assessment. It suggests that there is a definite association between poor sperm morphology and decreased pregnancy rates. Thus Sperm morphology evaluation is considered to be the best predictive factor in natural conception, IUI and an IVF procedure. Sperm morphology assessment by simple and inexpensive techniques can provide essential information similar to that obtained from some of the more complicated sperm function tests.
Diff-Quik
Diff-Quik staining is a relatively simple and a rapid staining method recommended by World Health organization(201O)
The staining kit consists of three solutions
1. Fixative 1X 50ml
2. Stain-I 1X 50ml
3. Stain-II 1X 50ml